猪GPR84和MPEG1基因的分子克隆和表达分析

doi:10.11843/j.issn.0366-6964.2014.05.004

Abstract

Abstract:

The aim of this study was to clone the full-length coding sequence (CDS) of pig GPR84 and MPEG1 genes，and to characterize their expression profiles in tissues and induced by poly(I:C) in cultured PK-15 cells at mRNA level.The Minzhu was used in this study.The full-length CDSs of GPR84 and MPEG1 genes were cloned with RT-PCR method,and analyzed by using bioinformatic method.The expression profiles were analyzed using real-time quantitative RT-PCR method.The result showed that the full-length CDS of GPR84 (GenBank accession No.JX280456) and MPEG1 （GenBank accession No.JQ907392）genes were 1 191 and 2 154 bp，encoding 396 and 717 aa，respectively.Both of the proteins shared more than 83% similarities with their respective orthologous in human and cattle.The GPR84 and MPEG1 genes were expressed in all the eleven tissues analyzed with different abundance.The GPR84 gene was expressed at the most abundant level in lung，while the MPEG1 gene in spleen.Both were expressed at the least abundant level in muscle.Additionally，the expression of GPR84 was induced by poly(I:C) in PK15 cells，while MPEG1 was suppressed.The results will lay a foundation for further revealing the roles of GPR84 and MPEG1 in porcine disease-resistant breeding.

CLC Number:

S828
S813.3

WANG Qiu-shi， WU Chun-yan， LI Peng， SHAO Si-yu， ZHANG Jiao， ZHU Meng， YANG Xiu-qin. Molecular Cloning and Expression Analysis of GPR84 and MPEG1 Genes in Porcine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 699-705.

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Molecular Cloning and Expression Analysis of GPR84 and MPEG1 Genes in Porcine

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